VTGM buiten de apotheek en toediening medicatie Module 2a Evidence-tabel

Cortopassi, 1977

Type of study: experimental study

Setting and country: laboratory, USA

Funding and conflicts of interest: no conflicts of interest.

Not applicable

Four experimental groups were set up:

1) isopropyl alcohol (70%) (n=40),

2) polymerized iodine solution (1%) (n=40),

3) water (n=40),

4) control (n=40)

Inoculated bags were scrubbed with either isopropyl alcohol (70%), polymerized iodine solution (1%), or water. Then, a sterile 5 mL disposable syringe was used to inject sterile water into the IV-bag via the additive site with was inoculated.

In part I, the additive ports of IV-bags (50 mL) containing 5% dextrose in water were inoculated with 0.01 mL of S. aureus containing 10⁷-10⁸ organisms.

In part II, the additive ports of IV-bags (50 mL) containing 5% dextrose in water were inoculated with 0.1 mL of S. aureus containing 10²-10³ organisms

Not applicable

Outcome measures and effect size (include 95%CI and p-value if available):

The IV-solution was then filtered to trap S. aureus, after which the filter was cultured.

Part I of the study (0.01 mL of S. aureus containing 10⁷-10⁸ organisms), isopropyl alcohol (2.5% contaminated, 1.00x10⁰ CFU/mL) performed significantly (p=0.05) better than the control group (100% contaminated, 3.78x10⁴ CFU/mL).

Part II of the study (0.1 mL of S. aureus containing 10²-10³ organisms), isopropyl alcohol (0% contaminated, 0 CFU/mL) again performed significantly (p=0.05) better than the control group (78% contaminated, 2.05x10² CFU/mL).